Jim Giovannoni

Professor, USDA Scientist
Jim Giovannoni
jjg33@cornell.edu
Office/Lab: 429/426
Phone: 607-254-1414
Office/Lab: 301
Email: jjg33@cornell.edu
Office Phone: 607-254-1414
Lab Phone: 607-255-1259
Affiliations: Adjunct Professor, Section of Plant Biology / School of Integrative Plant Science / Cornell University
Graduate Fields: Horticulture; Plant Breeding; Plant Biology
Research Overview

USDA affiliation: Plant Molecular Biologist, USDA-ARS Robert W. Holley Center james.giovannoni@ars.usda.gov

The focus of research in the Giovannoni laboratory is molecular and genetic analysis of fruit ripening and related signal transduction systems with emphasis on the relationship of fruit ripening to nutritional quality. We are also involved in development of tools for genomics of the Solanaceae including participation in the International Tomato Sequencing Project. We employ several experimental systems but the majority of our work involves the use of tomato. The broad objectives of the lab include deciphering the underlying molecular basis of components of ripening regulation conserved through evolution and how these regulatory networks coordinate ripening events including those related to quality and nutritional content.

Experimental approaches include: 1) positional cloning of loci known via mutation to harbor genes necessary for normal fruit development and ripening, and 2) isolation of candidate ripening regulatory genes based on expression pattern or relationship to ripening-related signal transduction systems (e.g. ethylene, light), and functional analysis in transgenic plants.

Intern Projects

Ripening is a process by which the texture, color, flavor, and nutritional content of fruit is enhanced. These traits contribute to the healthfulness and desirability of the fruit as a food source. Clearly, understanding the processes behind fruit ripening are important in terms of nutrition, but also for commercial applications such as transportation and shelf-life. Thus, the focus of research in the Giovannoni lab is molecular and genetic analysis of fruit ripening and related signal transduction systems, using tomato as the model system. Recently, researchers in the lab isolated two genes, RIN and NOR, that are part of the master switch to induce ripening in tomatoes. In addition to identifying important regulatory components of ripening, the lab also investigates lycopene production. Lycopene is the pigment that gives tomatoes their red coloring and which is also suggested to inhibit degenerative diseases such as cancer and heart disease. Using a genomics approach, the lab is investigating the regulatory mechanisms behind accumulation of this important compound in different tomato varieties.

For more information about the Giovannoni lab, please visit the Plant Biology website. Additionally, the Giovannoni lab, in conjunction with other labs on campus has developed a resource for tomato genomics, the Tomato EST Database. Additional resources and information resulting from tomato genomics activities on the Cornell campus can be found at the Solanaceae Genomics Network site.

Internship Program | Projects & FacultyApply for an Internship

Genetic and metabolic effects of ripening mutations and vine detachment on tomato fruit quality
2019.
Osorio, S., Carneiro, R.T., Lytovchenko, A., McQuinn, R., Sørensen, I., Vallarino, J.G., Giovannoni…
Plant Biotechnology Journal.
:
The tomato HIGH PIGMENT1/DAMAGED DNA BINDING PROTEIN 1 gene contributes to regulation of fruit ripening
2019.
Wang, A., Chen, D., Ma, Q., Rose, J.K.C., Fei, Zhangjun, Liu, Y., Giovannoni, James J.
Horticulture Research.
6
:
15
Plant Viruses Transmitted in Two Different Modes Produce Differing Effects on Small RNA-Mediated Processes in Their Aphid Vector
2019.
Pinheiro, P.V., Wilson, J.R., Xu, Y., Zheng, Y., Rebelo, A.R., Fattah-Hosseini, S., Kruse, A., Santo…
Phytobiomes.
3
:
71–81
Genome-wide association analysis identifies a natural variation in basic helix-loop-helix transcription factor regulating ascorbate biosynthesis via D-mannose/L-galactose pathway in tomato
2019.
Li, W., Ai, G., Li, C., Liu, G., Chen, W., Wang, B., Wang, W., Lu, Y., Zhang, J., Li, H., Ouyang, B.…
PLOS Genetics.
15
:
The tomato pan-genome uncovers new genes and a rare allele regulating fruit flavor
2019.
Gao, L., Gonda, I., Sun, H., Ma, Q., Bao, K., Tieman, D., Burzynski-Chang, E., Fish, T., Stromberg, …
Nature Genetics.
:
1546–1718
The Ptr1 locus of Solanum lycopersicoides confers resistance to race 1 strains of Pseudomonas syringae pv. tomato and to Ralstonia pseudosolanacearum by recognizing the type III effectors AvrRpt2/RipBN
2019.
Mazo-Molina, C., Mainiero, S., Hind, S.R., Kraus, C.M., Vachev, M., Maviane-Macia, F., Lindeberg, M…
Molecular Plant-Microbe Interactions.
:
Sequencing-Based Bin Map Construction of a Tomato Mapping Population, Facilitating High-Resolution Quantitative Trait Loci Detection
2018.
Gonda, I., Ashrafi, H., Lyon, D.A., Strickler, S.R., Hulse-Kemp, A.M., Ma, Q., Sun, H., Stoffel, K.,…
The Plant Genome.
12
:
Cucurbit Genomics Database (CuGenDB): a central portal for comparative and functional genomics of cucurbit crops
2018.
Zheng, Y., Wu, S., Bai, Y., Sun, H., Jiao, C., Guo, S., Zhao, K., Blanca, J., Zhang, Z., Huang, S., …
Nucleic Acids Research.
47
:
D1128–D1136
Natural genetic variation for expression of a SWEET transporter among wild species of Solanum lycopersicum (tomato) determines the hexose composition of ripening tomato fruit
2018.
Shammai, A., Petreikov, M., Yeselson, Y., Faigenboim, A., Moy‐Komemi, M., Cohen, S., Cohen, D., Be…
The Plant Journal.
96
:
343–357
HS-SPME-GC-MS Analyses of Volatiles in Plant Populations—Quantitating Compound × Individual Matrix Effects
2018.
Burzynski-Chang, E., Ryona, I., Reisch, B., Gonda, I., Foolad, M., Giovannoni, James, Sacks, G.
Molecules.
23
:
2436
Tomato Multiomics Reveals Consequences of Crop Domestication and Improvement
2018.
Giovannoni, James
Cell.
172
:
6–8
Genome encode analyses reveal the basis of convergent evolution of fleshy fruit ripening
2018.
Lü, P., Yu, S., Zhu, N., Chen, Y., Zhou, B., Pan, Y., Tzeng, D., Fabi, J.P., Argyris, J., Garcia-M…
Nature Plants.
4
:
784–791
MaMADS2 repression in banana fruits modifies hormone synthesis and signalling pathways prior to climacteric stage
2018.
Yakir, E., Fei, Zhangjun, Sela, N., Xu, Y., Singh, V., Dagar, A., Joshi, J.R., Müller, M., Munné-…
BMC Plant Biology.
18
:
1471–2229
Ectopic expression of ORANGE promotes carotenoid accumulation and fruit development in tomato
2018.
Yazdani, M., Sun, Z., Yuan, H., Zeng, S., Thannhauser, T. W., Vrebalov, J., Ma, Q., Xu, Y., Fei, Zha…
Plant Biotechnology Journal.
:
Genetic mapping identifies loci that influence tomato resistance against Colorado potato beetles
2018.
Vargas-Ortiz, E., Gonda, I., Smeda, J. R., Mutschler, M. A., Giovannoni, James J., Jander, Georg
Nature Scientific Reports.
8
:
Genetic diversity within African tomato using next generation sequencing
2018.
Mungai, G. W., Owino, W., Ambuko, J., Giovannoni, James J., Nyende, A. B., Michuki, G.
PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION.
:
1–10
Deciphering Genetic Factors that Determine Melon Fruit-Quality Traits Using RNA-Seq-Based High-Resolution QTL and eQTL Mapping
2018.
Galpaz, N., Gonda, I., Shem-Tov, D., Barad, O., Tzuri, G., Lev, S., Fei, Zhangjun, Xu, Y., Lombardi,…
The Plant Journal.
94
:
169–191
Differential metabolism of L–phenylalanine in the formation of aromatic volatiles in melon (Cucumis melo L.) fruit
2018.
Gonda, I., Davidovich-Rikanati, R., Bar, E., Lev, S., Jhirad, P., Meshulam, Y., Wissotsky, G., Portn…
Phytochemistry.
148
:
122–131
High-resolution spatiotemporal transcriptome mapping of tomato fruit development and ripening
2018.
Shinozaki, Y., Nicolas, P., Fernandez-Pozo, N., Ma, Q., Evanich, D. J., Shi, Y., Xu, Y., Zheng, Y., …
Nature Communications.
9
:
Potato leafroll virus alters small RNA production in its aphid vector during plant viral infection to promote virus dispersal
2017.
Wilson, J., Pinheiro, P., Xu, Y., Zheng, Y., Rebelo, A. R., Fattahalhosseini, S., Kruse, A., Dos Sil…
PHYTOPATHOLOGY.
107
:
206–207

Rin gene compositions and methods for use thereof
Jim Giovannoni
Technology Area:Fruit Quality
US Patent/Application(s): 6,787,687
Publication: Science 2002
Ripening inhibition in the tomato Green-ripe mutant results from ectopic expression of a novel protein which disrupts ethylene signal transduction
Jim Giovannoni
Technology Area:Fruit Quality
US Patent/Application(s): 7,947,867
Publication: PNAS 2006
NOR gene for use in manipulation of fruit quality and ethylene response
Jim Giovannoni
Technology Area:Fruit Quality
US Patent/Application(s): 6,762,347
Publication: Plant Cell 2004
Endo-1,4-.beta.-glucanase genes and their use in plants
Jim Giovannoni
Technology Area:Fruit Quality
US Patent/Application(s): 5,585,545
Publication: Plant J 1998
Banana MADS-Box Genes for Banana Ripening Control
Jim Giovannoni
Technology Area:Fruit Quality
US Patent/Application(s): Provisional
Publication: J Exp Bot 2010
 

Contact:

Boyce Thompson Institute
533 Tower Rd.
Ithaca, NY 14853
607.254.1234
contact@btiscience.org