David Stern

David Stern
ds28@cornell.edu
Office/Lab: 120/213
Phone: 607-254-1300
David Stern standing at his farm
Email: ds28@cornell.edu
Office/Lab: 120/213
Office Phone: 607-254-1300
Lab Phone: 607-254-1304
Affiliations: Section of Plant Biology / School of Integrative Plant Science / Cornell University
Graduate Fields: Plant Biology; Genetics & Development; Biochemistry, Cell & Molecular Biology
Research Areas:  Stress Responses / Cell Biology
Research Overview

The underlying research themes in the Stern laboratory are chloroplast biology, bioenergy and nuclear-cytoplasmic interactions. Within this framework, we study how chloroplast genes and metabolic activities are regulated by the products of nuclear genes, usually acting at the transcriptional or post-transcriptional level. Areas of emphasis include the roles of ribonucleases and RNA-binding proteins and assembly of the carbon-fixing enzyme Rubisco. We are also using molecular and genetic techniques to adjust chloroplast metabolism for the production of useful hydrocarbons.

Intern Projects

The underlying research themes in the Stern laboratory are chloroplast biology, bioenergy and nuclear-cytoplasmic interactions. Within this framework, we study how chloroplast genes and metabolic activities are regulated by the products of nuclear genes, usually acting at the transcriptional or post-transcriptional level. Areas of emphasis include the roles of ribonucleases and RNA-binding proteins and assembly of the carbon-fixing enzyme Rubisco. We are also using molecular and genetic techniques to adjust chloroplast metabolism for the production of useful hydrocarbons.

Internship Program | Projects & FacultyApply for an Internship

Rubisco production in maize mesophyll cells through ectopic expression of subunits and chaperones
2021.
Hotto, A.M., Salesse-Smith, C., Lin, Y., Busch, F.A., Simpson, I., Stern, David
J Exp Bot..
:
Increased Rubisco content in maize mitigates chilling stress and speeds recovery
2020.
Salesse-Smith, C.E., Sharwood, R.E., Busch, F.A., Stern, David
:
CFM1, a Member of the CRM-domain Protein Family, Functions in Chloroplast Group II Intron Splicing in Setaria viridis
2020.
Feiz, L., Asakura, Y., Mao, L., Strickler, Susan R., Fei, Zhangjun, Rojas, M., Barkan, A., Stern, Da…
:
Plant science decadal vision 2020-2030: Reimagining the potential of plants for a healthy and sustainable future
2020.
Henkhaus, N., Barlett, M., Gang, D., Grumet, R., Jordon-Thaden, I., Lorence, A., Lyons, E., Miller, …
Plant Direct..
4
:
Setaria viridis chlorotic and seedling-lethal mutants define critical functions for chloroplast gene expression
2020.
Feiz, L., Strickler, S.R., Van Eck, Joyce, Mao, L., Movahed, N., Taylor, C., Gourabathini, P., Fei, …
Plant J..
:
Systematic Sequencing of Chloroplast Transcript Termini From Arabidopsis Thaliana Reveals >200 Transcription Initiation Sites and the Extensive Imprints of RNA-binding Proteins and Secondary Structures
2020.
Castandet, B., Germain, A., Hotto, A.M., Stern, David
Nucelic Acids Res..
:
Plant Ribonuclease J: An Essential Player in Maintaining Chloroplast RNA Quality Control for Gene Expression.
2020.
Hotto, A.M.,, Stern, David, Schuster, G.
Plants.
9(3)
:
Systematic sequencing of chloroplast transcript termini from Arabidopsis thaliana reveals >200 transcription initiation sites and the extensive imprints of RNA-binding proteins and secondary structures
2019.
Castandet, B., Germain, A., Hotto, A.M., Stern, David
Nucleic Acids Research.
47
:
11889–11905
Increased Rubisco content in maize mitigates chilling stress and speeds recovery
2019.
Salesse‐Smith, C., Sharwood, R., Busch, F., Stern, David
Plant Biotechnology Journal.
:
1–12
Directions for research and training in plant omics: Big Questions and Big Data
2019.
Argueso, C.T., Assmann, S.M., Birnbaum, K.D., Chen, S., Dinneny, J.R., Doherty, C.J., Eveland, A.L.,…
Plant Direct.
:
Overexpression of Rubisco subunits with RAF1 increases Rubisco content in maize
2018.
Salesse-Smith, C.E., Sharwood, R.E., Busch, F.A., Kromdijk, J., Bardal, V. and Stern, David
Nature Plants.
4
:
802–810
Reinventing postgraduate training in the plant sciences: T‐training defined through modularity, customization, and distributed mentorship
2018.
Henkhaus, N.A., Taylor, C.B., Greenlee, V.R., Sickler, D.B., Stern, David
Plant Direct.
2
:
e00095
A Guide to the Chloroplast Transcriptome Analysis Using RNA-Seq
2018.
Michel, E. JS, Hotto, A. M., Strickler, S. R., Stern, David, Castandet, B.
The Nucleus (Methods in Molecular Biology).
1829
:
295–313
High‐throughput droplet microfluidics screening platform for selecting fast‐growing and high lipid‐producing microalgae from a mutant library
2017.
Kim, H.S., Hsu, S.‐C., Han, S.‐I., Thapa, H.R., Guzman, A.R., Browne, D.R., Tatli, M., Devarenne…
Plant Direct.
1
:
e00011
The Rubisco Chaperone BSD2 May Regulate Chloroplast Coverage in Maize Bundle Sheath Cells
2017.
Salesse-Smith, C., Sharwood, R. E., Sakamoto, W., Stern, David
Plant Physiology.
175
:
pp.01346.2017–pp.01346.2017
Selecting high-growth/high-lipid producing microalgae from a mutant library through droplet microfluidics
2016.
Kim, H. S., Han, S., Guzman, A. R., Sobahi, N., Thapa, H. R., Browne, D., Tatli, M., Hsu, S., Stern,…
:
200–201
ChloroSeq, an Optimized Chloroplast RNA-Seq Bioinformatic Pipeline, Reveals Remodeling of the Organellar Transcriptome Under Heat Stress
2016.
Castandet, B., Hotto, A. M., Strickler, S. R., Stern, David
G3: Genes|Genomes|Genetics.
6
:
2817–2827
Activation of an Endoribonuclease by Non-intein Protein Splicing
2016.
Campbell, S. J., Stern, David
Journal of Biological Chemistry.
291
:
15911–15922
Arabidopsis Chloroplast Mini-Ribonuclease III Participates in rRNA Maturation and Intron Recycling
2015.
Hotto, A. M., Castandet, B., Gilet, L., Higdon, A., Condon, C., Stern, David
The Plant Cell.
27
:
724–740
A protein with an inactive pterin-4a-carbinolamine dehydratase domain is required for Rubisco biogenesis in plants
2014.
Feiz, L., Williams-Carrier, R., Belcher, S., Montano, M., Barkan, A., Stern, David
The Plant Journal.
80
:
862–869
 

Contact:

Boyce Thompson Institute
533 Tower Rd.
Ithaca, NY 14853
607.254.1234
contact@btiscience.org