David Stern

Professor, President
David Stern
ds28@cornell.edu
Office/Lab: 301/213
Phone: 607-254-1300
Email: ds28@cornell.edu
Office/Lab: 301/213
Office Phone: 607-254-1300
Lab Phone: 607-254-1304
Affiliations: Section of Plant Biology / School of Integrative Plant Science / Cornell University
Graduate Fields: Plant Biology; Genetics & Development; Biochemistry, Cell & Molecular Biology
Research Areas:  Stress Responses / Cell Biology
Research Overview

The underlying research themes in the Stern laboratory are chloroplast biology, bioenergy and nuclear-cytoplasmic interactions. Within this framework, we study how chloroplast genes and metabolic activities are regulated by the products of nuclear genes, usually acting at the transcriptional or post-transcriptional level. Areas of emphasis include the roles of ribonucleases and RNA-binding proteins and assembly of the carbon-fixing enzyme Rubisco. We are also using molecular and genetic techniques to adjust chloroplast metabolism for the production of useful hydrocarbons.

Intern Projects
The underlying research themes in the Stern laboratory are chloroplast biology, bioenergy and nuclear-cytoplasmic interactions. Within this framework, we study how chloroplast genes and metabolic activities are regulated by the products of nuclear genes, usually acting at the transcriptional or post-transcriptional level. Areas of emphasis include the roles of ribonucleases and RNA-binding proteins and assembly of the carbon-fixing enzyme Rubisco. We are also using molecular and genetic techniques to adjust chloroplast metabolism for the production of useful hydrocarbons.

Internship Program | Projects & FacultyApply for an Internship
Arabidopsis Chloroplast Mini-Ribonuclease III Participates in rRNA Maturation and Intron Recycling
2015
Author(s):Hotto, A.M., Castandet, B., Gilet, L., Higdon, A., Condon, C., and Stern, D.B.
The Plant Cell
tpc.114.134452
View
A protein with an inactive pterin-4a-carbinolamine dehydratase domain is required for Rubisco biogenesis in plants
2014
Author(s):Feiz, L., Williams-Carrier, R., Belcher, S., Montano, M., Barkan, A, and Stern, D.B.
Plant Journal
80,
862-869
View
RNase J participates in a pentatricopeptide repeat protein-mediated 5‰€_ end maturation of chloroplast mRNAs.
2013
Author(s):Luro, S., Germain, A., Sharwood, R.E., and Stern, D.B.
Nucleic Acids Research
41
9141-9151
View
Strand-specific RNA sequencing uncovers chloroplast ribonuclease functions
2013
Author(s):Castandet, B., Hotto, A.M., Fei, Z., and Stern, D.B.
FEBS Lett.
587,
3096-3101
View
RNA processing and decay in plastids
2013
Author(s):Germain, A., Hotto, A.M., Barkan, A., and Stern, D.B.
Wiley Interdisciplinary Reviews: RNA
4,
295-316
View
RNase J participates in a pentatricopeptide repeat protein-mediated 5′ end maturation of chloroplast mRNAs.
2013
Author(s):Luro, S., Germain, A., Sharwood, R.E., and Stern, D.B.
Nucleic Acids Research
41,
9141-9151
View
Arabidopsis chloroplast quantitative editotype
2013
Author(s):Ruwe, H., Castandet, B., Schmitz-Linneweber, C., and Stern, D.B.
FEBS Lett.
587,
1429-1433
View
Ribonuclease II preserves chloroplast RNA homeostasis by increasing mRNA decay rates, and cooperates with polynucleotide phosphorylase in 3‰€_ end maturation
2012
Author(s):Germain, A., Kim, S.H., Gutierrez, R., and Stern, D.B.
Plant J.
72
960-971
View
Ribulose-1,5-bis-phosphate carboxylase/oxygenase accumulation factor1 is required for holoenzyme assembly in maize
2012
Author(s):Feiz, L., Williams-Carrier, R., Wostrikoff, K., Belcher, S., Barkan, A., and Stern, D.B.
Plant Cell
24,
3435-3446
View
Ribonuclease II preserves chloroplast RNA homeostasis by increasing mRNA decay rates, and cooperates with polynucleotide phosphorylase in 3′ end maturation
2012
Author(s):Germain, A., Kim, S.H., Gutierrez, R., and Stern, D.B.
Plant J.
72,
960-971
View
Plastid non-coding RNAs: emerging candidates for gene regulation
2012
Author(s):Hotto , A.M., Germain, A., and Stern, D.B.
Trends Plant Sci.
17,
737-744
View
Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3‰€_-end maturation and intron degradation
2011
Author(s):Germain, A., Herlich, S., Larom, S., Kim, S.H., Schuster, G., and Stern, D.B.
Plant J.
67
381-394
View
Overaccumulation of the chloroplast antisense RNA AS5 is correlated with decreased abundance of 5S rRNA in vivo and inefficient 5S rRNA maturation in vitro.
2011
Author(s):Sharwood, R.E., Hotto, A.M., Bollenbach, T.J., and Stern, D.B.
RNA
17,
230-243
View
Chloroplast RNase J compensates for inefficient transcription termination by removal of antisense RNA
2011
Author(s):Sharwood, R.E., Halpert, M., Luro, S., Schuster, G., and Stern, D.B.
RNA
17,
2165-2176
View
Unexpected diversity of chloroplast noncoding RNAs as revealed by deep sequencing of the Arabidopsis transcriptome G3
2011
Author(s):Hotto, A.M., Schmitz, R.J., Fei, Z., Ecker, J.E., and Stern, D.B.
G3 (Bethesda)
1(7),
559–570
View
Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3′-end maturation and intron degradation
2011
Author(s):Germain, A., Herlich, S., Larom, S., Kim, S.H., Schuster, G., and Stern, D.B.
Plant J.
67,
381-394
View
Differential replication of two chloroplast genome forms in heteroplasmic Chlamydomonas reinhardtii gametes contributes to alternative inheritance patterns.
2010
Author(s):Nishimura, Y., and Stern, D.B.
Genetics
185,
1167-1181
View
MRL1, a Conserved Pentatricopeptide Repeat Protein, Is Required for Stabilization of rbcL mRNA in Chlamydomonas and Arabidopsis
2010
Author(s):Johnson, X., Wostrikoff, K., Finazzi, G., Kuras, R., Schwarz, C., Bujaldon, S., Nickelsen, J., Stern, D.B., Wollman, F.A., and Vallon, O.
Plant Cell
22,
234-248
View
Overexpression of a natural chloroplast-encoded antisense RNA in tobacco destabilizes 5S rRNA and retards plant growth
2010
Author(s):Hotto, A.M., Huston, Z.E., and Stern, D.B.
, 10, 213.
10,
213
View
Insights into the evolution of mitochondrial genome size from complete sequences of Citrullus lanatus and Cucurbita pepo (Cucurbitaceae)
2010
Author(s):Alverson, A.J., Wei, X.X., Rice, D.W., Stern, D.B., Barry, K., and Palmer, J.D.
Molecular Biology and Evolution
27,
1436-1448
View
Transgenic maize lines with cell-type specific expression of fluorescent proteins in plastids
2010
Author(s):Sattarzadeh, A., Fuller, J., Moguel, S., Wostrikoff, K., Sato, S., Covshoff, S., Clemente, T., Hanson, M., and Stern, D.B.
Plant Biotechnology Journal
8,
112-125
View
Chloroplast RNA metabolism
2010
Author(s):Stern, D.B., Goldschmidt-Clermont, M., and Hanson, M.R.
Annu. Rev. Plant Biol.
61,
125-155
View
Use of Illumina sequencing to identify transposon insertions underlying mutant phenotypes in high-copy Mutator lines of maize
2010
Author(s):Williams-Carrier, R., Stiffler, N., Belcher, S., Kroeger, T., Stern, D.B., Monde, R.A., Coalter, R., and Barkan, A.
Plant J.
6,
167-177
View
The RNA-binding proteins CSP41a and CSP41b may regulate transcription and translation of chloroplast-encoded RNAs in Arabidopsis
2009
Author(s):Bollenbach, T.J., Sharwood, R.E., Gutierrez, R., Lerbs-Mache, S., and Stern, D.B.
Plant Molecular Biology
69,
541-552
View
Mitochondria and Chloroplasts
2009
Author(s):Newton, K.J., Stern, D.B., and Gabay-Laughnan, S.
In Handbook of Maize
(Bennetzen, J.L. and Hake, S.C. eds),
0: Springer
View
Abnormal physiological and molecular mutant phenotypes link chloroplast polynucleotide phosphorylase to the phosphorus deprivation response in Arabidopsis
2009
Author(s):Marchive, C., Yehudai-Resheff, S., Germain, A., Fei, Z.J., Jiang, X.S., Judkins, J., Wu, H., Fernie, A.R., Fait, A., and Stern, D.B.
Plant Physiology
151,
905-924
View
Polyadenylation in Arabidopsis and Chlamydomonas organelles: the input of nucleotidyltransferases, poly(A) polymerases and polynucleotide phosphorylase
2009
Author(s):Zimmer, S.L., Schein, A., Zipor, G., Stern, D.B., and Schuster, G.
Plant J.
59,
88-99
View
Organellar and metabolic processes
2008
Author(s):Stern, D.B.
In The Chlamydomonas Sourcebook
(Stern, D.B. ed.),
Oxford 0: Elsevier
View
Nuclear, chloroplast, and mitochondrial transcript abundance along a maize leaf developmental gradient
2008
Author(s):Cahoon, A.B., Takacs, E.M., Sharpe, R.M., and Stern, D.B.
Plant Mol. Biol.
66,
33-46
View
Genome-based analysis of Chlamydomonas reinhardtii exoribonucleases and poly(A) polymerases predicts unexpected organellar and exosomal features
2008
Author(s):Zimmer, S.L., Fei, Z.J., and Stern, D.B.
Genetics
179,
125-136
View
Integration of chloroplast nucleic acid metabolism into the phosphate deprivation response in Chlamydomonas reinhardtii
2007
Author(s):Yehudai-Resheff, S., Zimmer, S.L., Komine, Y., Stern, D.B.
Plant Cell
19,
1023-1038
View
Rubisco large subunit translation is autoregulated in response to its assembly state in tobacco chloroplasts
2007
Author(s):Wostrikoff, K., and Stern, D.B.
P. Natl. Acad. Sci. U S A
104,
6466-6471
View
Nuclear Suppressors Define Three Factors that Participate in Both 5‰€™ and 3‰€™ End Processing of mRNAs in Chlamydomonas chloroplasts
2006
Author(s):Rymarquis, L.A., Higgs, D.C., and Stern, D.B.
Plant J.
46
448-461
View
Nuclear Suppressors Define Three Factors that Participate in Both 5’ and 3’ End Processing of mRNAs in Chlamydomonas chloroplasts
2006
Author(s):Rymarquis, L.A., Higgs, D.C., and Stern, D.B.
Plant J.
46,
448-461
View
Adaptive Evolution of Chloroplast Genome Structure Inferred Using a Parametric Bootstrap Approach
2006
Author(s):Cui, L., Leebens-Mack, J., Wang, L.S., Tang, J., Rymarquis, L., Stern, D.B., and dePamphilis, C.W.
BMC Evol. Biol.
6,
13
View
Chlamydomonas reinhardtii Encodes a Single sigma70-like Factor Which Likely Functions in Chloroplast Transcription
2006
Author(s):Bohne, A.V., Ihrimovitch, V., Weihe, A., and Stern, D.B.
Curr. Genet.
49,
333-340
View
The sulfur acclimation SAC3 kinase is required for chloroplast transcriptional repression under sulfur limitation inChlamydomonas reinhardtii
2006
Author(s):Irihimovitch, V., and Stern, D.B.
P. Natl. Acad. Sci. U S A
103,
7911-7916
View
RNR1, a 3‰€™-5‰€™ Exoribonuclease Belonging to the RNR Superfamily, Catalyzes 3‰€™ Maturation of Chloroplast Ribosomal RNAs in Arabidopsis thaliana
2005
Author(s):Bollenbach, T.J., Lange, H., Gutierrez, R., Stern, D.B., and Gagliardi, D.
Nucleic Acids Research
33
2751-2763
View
Beyond Complementation: Map-Based Cloning in Chlamydomonas reinhardtii
2005
Author(s):Rymarquis, L.A., Handley, J.M., Thomas, M., and Stern, D.B.
Plant Physiology
137,
557-566
View
A Spontaneous tRNA Suppressor of a Mutation in the Chlamydomonas reinhardtii Nuclear MCD1 Gene Required for Stability of the Chloroplast petD mRNA
2005
Author(s):Murakami, S., Kuehnle, K., and Stern, D.B.
Nucleic Acids Research
33,
3372-3380
View
Microarray Analysis Confirms the Specificity of a Chlamydomonas reinhardtii Chloroplast RNA Stability Mutant
2005
Author(s):Erickson, B., Stern, D.B., and Higgs, D.C
Plant Physiology
137,
534-544
View
RNR1, a 3’-5’ Exoribonuclease Belonging to the RNR Superfamily, Catalyzes 3’ Maturation of Chloroplast Ribosomal RNAs in Arabidopsis thaliana
2005
Author(s):Bollenbach, T.J., Lange, H., Gutierrez, R., Stern, D.B., and Gagliardi, D.
Nucleic Acids Research
33,
2751-2763
View
Genetics and Genomics of Chloroplast Biogenesis: Maize as a Model System
2004
Author(s):Stern, D.B., Hanson, M.R., and Barkan, A.
Trends in Plant Science
9,
293-301
View
Antisense Transcript and RNA Processing Alterations Suppress Instability of Polyadenylated mRNA in Chlamydomonas chloroplasts
2004
Author(s):Nishimura, Y., Kikis, E.A., Zimmer, S.L., Komine, Y., and Stern, D.B.
Plant Cell
16,
2849-2869
View