David Stern

President
David Stern
ds28@cornell.edu
Office/Lab: 120/213
Phone: 607-254-1300
David Stern standing at his farm
Email: ds28@cornell.edu
Office/Lab: 120/213
Office Phone: 607-254-1300
Lab Phone: 607-254-1304
Affiliations: Section of Plant Biology / School of Integrative Plant Science / Cornell University
Graduate Fields: Plant Biology; Genetics & Development; Biochemistry, Cell & Molecular Biology
Research Areas:  Stress Responses / Cell Biology
Research Overview

The Stern laboratory focuses on photosynthetic carbon assimilation and gene expression mechanisms in the chloroplast, where photosynthesis takes place. We use molecular genetic techniques to test hypotheses for increasing plant performance and photosynthetic efficiency by creating and analyzing transgenic plants. Our gene expression work focuses on a key ribonuclease, RNase J, which exerts quality control over the transcript population of the chloroplast, and is itself essential for plant embryo development and viability. 

Intern Projects
The Stern laboratory focuses on photosynthetic carbon assimilation and gene expression mechanisms in the chloroplast, where photosynthesis takes place. We use molecular genetic techniques to test hypotheses for increasing plant performance and photosynthetic efficiency by creating and analyzing transgenic plants. Our gene expression work focuses on a key ribonuclease, RNase J, which exerts quality control over the transcript population of the chloroplast, and is itself essential for plant embryo development and viability.

Internship Program | Projects & FacultyApply for an Internship
Rubisco production in maize mesophyll cells through ectopic expression of subunits and chaperones
2021.
Hotto, A.M., Salesse-Smith, C., Lin, Y., Busch, F.A., Simpson, I., Stern, David
J Exp Bot..
:
Increased Rubisco content in maize mitigates chilling stress and speeds recovery
2020.
Salesse-Smith, C.E., Sharwood, R.E., Busch, F.A., Stern, David
:
CFM1, a Member of the CRM-domain Protein Family, Functions in Chloroplast Group II Intron Splicing in Setaria viridis
2020.
Feiz, L., Asakura, Y., Mao, L., Strickler, Susan R., Fei, Zhangjun, Rojas, M., Barkan, A., Stern, David
:
Plant science decadal vision 2020-2030: Reimagining the potential of plants for a healthy and sustainable future
2020.
Henkhaus, N., Barlett, M., Gang, D., Grumet, R., Jordon-Thaden, I., Lorence, A., Lyons, E., Miller, S., Murray, S., Nelson, Andrew, Li, Fay-Wei, Sickler, Delanie, Taylor, C., Stern, David
Plant Direct..
4
:
Setaria viridis chlorotic and seedling-lethal mutants define critical functions for chloroplast gene expression
2020.
Feiz, L., Strickler, S.R., Van Eck, Joyce, Mao, L., Movahed, N., Taylor, C., Gourabathini, P., Fei, Zhangjun, Stern, David
Plant J..
:
Systematic Sequencing of Chloroplast Transcript Termini From Arabidopsis Thaliana Reveals >200 Transcription Initiation Sites and the Extensive Imprints of RNA-binding Proteins and Secondary Structures
2020.
Castandet, B., Germain, A., Hotto, A.M., Stern, David
Nucelic Acids Res..
:
Plant Ribonuclease J: An Essential Player in Maintaining Chloroplast RNA Quality Control for Gene Expression.
2020.
Hotto, A.M.,, Stern, David, Schuster, G.
Plants.
9(3)
:
Systematic sequencing of chloroplast transcript termini from Arabidopsis thaliana reveals >200 transcription initiation sites and the extensive imprints of RNA-binding proteins and secondary structures
2019.
Castandet, B., Germain, A., Hotto, A.M., Stern, David
Nucleic Acids Research.
47
:
11889–11905
Increased Rubisco content in maize mitigates chilling stress and speeds recovery
2019.
Salesse‐Smith, C., Sharwood, R., Busch, F., Stern, David
Plant Biotechnology Journal.
:
1–12
Directions for research and training in plant omics: Big Questions and Big Data
2019.
Argueso, C.T., Assmann, S.M., Birnbaum, K.D., Chen, S., Dinneny, J.R., Doherty, C.J., Eveland, A.L., Friesner. J., Greenlee, V.R., Law, J.A., Marshall-Colón, A., Mason, G.A., O'Lexy, R., Peck, S.C., Schmitz, R.J., Song, L., Stern, David, Varagona, M.J., Walley, J.W., Williams, C.M.
Plant Direct.
:
Overexpression of Rubisco subunits with RAF1 increases Rubisco content in maize
2018.
Salesse-Smith, C.E., Sharwood, R.E., Busch, F.A., Kromdijk, J., Bardal, V. and Stern, David
Nature Plants.
4
:
802–810
Reinventing postgraduate training in the plant sciences: T‐training defined through modularity, customization, and distributed mentorship
2018.
Henkhaus, N.A., Taylor, C.B., Greenlee, V.R., Sickler, D.B., Stern, David
Plant Direct.
2
:
e00095
A Guide to the Chloroplast Transcriptome Analysis Using RNA-Seq
2018.
Michel, E. JS, Hotto, A. M., Strickler, S. R., Stern, David, Castandet, B.
The Nucleus (Methods in Molecular Biology).
1829
:
295–313
High‐throughput droplet microfluidics screening platform for selecting fast‐growing and high lipid‐producing microalgae from a mutant library
2017.
Kim, H.S., Hsu, S.‐C., Han, S.‐I., Thapa, H.R., Guzman, A.R., Browne, D.R., Tatli, M., Devarenne, T.P., Stern, David B., Han, A.
Plant Direct.
1
:
e00011
The Rubisco Chaperone BSD2 May Regulate Chloroplast Coverage in Maize Bundle Sheath Cells
2017.
Salesse-Smith, C., Sharwood, R. E., Sakamoto, W., Stern, David
Plant Physiology.
175
:
pp.01346.2017–pp.01346.2017
Selecting high-growth/high-lipid producing microalgae from a mutant library through droplet microfluidics
2016.
Kim, H. S., Han, S., Guzman, A. R., Sobahi, N., Thapa, H. R., Browne, D., Tatli, M., Hsu, S., Stern, David, Devarenne, T. P., Han, A.
:
200–201
ChloroSeq, an Optimized Chloroplast RNA-Seq Bioinformatic Pipeline, Reveals Remodeling of the Organellar Transcriptome Under Heat Stress
2016.
Castandet, B., Hotto, A. M., Strickler, S. R., Stern, David
G3: Genes|Genomes|Genetics.
6
:
2817–2827
Activation of an Endoribonuclease by Non-intein Protein Splicing
2016.
Campbell, S. J., Stern, David
Journal of Biological Chemistry.
291
:
15911–15922
Arabidopsis Chloroplast Mini-Ribonuclease III Participates in rRNA Maturation and Intron Recycling
2015.
Hotto, A. M., Castandet, B., Gilet, L., Higdon, A., Condon, C., Stern, David
The Plant Cell.
27
:
724–740
A protein with an inactive pterin-4a-carbinolamine dehydratase domain is required for Rubisco biogenesis in plants
2014.
Feiz, L., Williams-Carrier, R., Belcher, S., Montano, M., Barkan, A., Stern, David
The Plant Journal.
80
:
862–869

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