Greg Martin

Boyce Schulze Downey Professor
Greg Martin
gbm7@cornell.edu
Office/Lab: 327/326
Phone: 607-254-1208
Office/Lab: 327/326
Email: gbm7@cornell.edu
Office Phone: 607-254-1208
Lab Phone: 607-220-9610
Graduate Fields: Plant Pathology & Plant-Microbe Biology; Plant Biology
Research Overview

How do bacteria infect plants and how do plants defend themselves from such attacks?

The long-term goal of research in the Martin laboratory is to use knowledge gained about the molecular basis of plant-pathogen interactions to develop plants with increased natural resistance to diseases. Such plants would require fewer applications of pesticides producing economic and environmental benefits while providing food for consumers with less pesticide residue.

The Martin laboratory studies the molecular basis of bacterial infection processes and the plant immune system. The research focuses on speck disease which is caused by the infection of tomato leaves with the bacterial pathogen Pseudomonas syringae pv. tomato. This is an economically important disease that can decrease both the yield and quality of tomato fruits. It also serves as an excellent experimental system for studying the molecular mechanisms that underlie plant-pathogen interactions and how they have evolved. Many experimental resources, including an increasing number of genome sequences, are available for both tomato and P. s. pv. tomato. Current work relies on diverse experimental approaches involving methods derived from the fields of biochemistry, bioinformatics, cell biology, forward and reverse genetics, genomics, molecular biology, plant breeding, plant pathology and structural biology.

Research in the Martin lab is supported, in part, by: USDA-BARD IS-4931-16C, NSF IOS-1451754 and NSF-IOS-1546625

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In the interaction of Pseudomonas with tomato, the plant responds rapidly to a potential infection by detecting certain conserved molecules expressed by the pathogen. At this stage, the pathogen uses a specialized secretion system to deliver virulence proteins, such as AvrPto and AvrPtoB, into the plant cell. These pathogen proteins suppress early host defenses and thereby promote disease susceptibility. Some tomato varieties express a resistance gene, Pto, which encodes a protein that detects the presence of AvrPto or AvrPtoB and activates a second strong immune system that halts the progression of bacterial speck disease.

The Martin lab is currently studying many aspects of the molecular mechanisms that underlie the bacterial infection process and the plant response to infection. One project takes advantage of the genetic natural variation present in wild relatives of tomato to identify new genes that contribute to plant immunity. These genes provide insights into the plant immune system and also can be bred into new tomato varieties to enhance disease resistance. A second project relies on next-generation sequencing methods to identify tomato genes whose expression increases during the interaction with P. s. pv. tomato. The expression of these genes is then reduced by using virus-induced gene silencing or the genes are mutated using CRISPR/Cas9 to test whether they make a demonstrable contribution to immunity.

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Martin Lab Pedigree

Evolution of immunity

Evolution of pathogen recognition and defense responses in wild species of tomato and tomato heirloom varieties.

Pattern-triggered immunity

The plant immune system 1: Mechanisms underlying recognition and response to microbe-associated molecular patterns.

Effector-triggered immunity

The plant immune system II: Mechanisms underlying recognition and response to pathogen effector proteins.

AvrPto virulence mechanisms

Virtulence mechanisms of the AvrPto effector protein from Pseudomonas syringae pv. tomato.

Methods to study plant immunity

Development of methods and resources to study the plant immune system.

Nicotiana benthamiana genome project

Nicotiana benthamiana genome project.

Intern Projects
Investigating pathogen virulence mechanisms using novel isolates of Pseudomonas syringae pv. tomato collected during a recent outbreak of bacterial speck disease in New York.  Identifying and characterizing immunity-associated genes from wild relatives of tomato.

The Martin laboratory studies the molecular basis of plant immunity and bacterial pathogenesis. Our focus is on the infection of tomato by Pseudomonas syringae pv. tomato as this process results in bacterial speck, an economically important disease, and also serves as a powerful model system for understanding fundamental mechanisms involved in plant-pathogen interactions. On the plant side, we identify and characterize genes, proteins and molecular mechanisms that play a role in host immunity and susceptibility. This work relies on natural variation for these traits that is present in cultivated tomato and in the 12 wild relatives of tomato that occur in South America. On the bacterial side, we study the proteins and associated mechanisms that the pathogen uses to interfere with the plant immune response. For the characterization of both plant and bacterial genes and proteins, we use a variety of experimental approaches including biochemistry, bioinformatics, genetics, genomics, molecular biology, and structural biology.

Examples of research projects for undergraduates in my laboratory include: 1) molecular characterization of novel isolates of Pseudomonas syringae pv. tomato collected during a recent outbreak of bacterial speck disease in New York; and 2) Identifying and characterizing immunity-associated genes from wild relatives of tomato. For more information about the Martin lab, please visit the various sections on this page or the Plant Pathology website.

Interns in the Martin Lab are funded by NSF IOS-1451754 and REU-1358843.

Internship Program | Projects & FacultyApply for an Internship
The Tomato Kinase Pti1 Contributes to Production of Reactive Oxygen Species in Response to Two Flagellin-Derived Peptides and Promotes Resistance to Pseudomonas syringae Infection
2017.
Schwizer, S., Kraus, C. M., Dunham, D. M., Zheng, Y., Fernandez-Pozo, N., Pombo, M. A., Fei, Zhangju…
Molecular plant-microbe interactions : MPMI.
30
:
725–738
A Subset of Ubiquitin-Conjugating Enzymes Is Essential for Plant Immunity
2017.
Zhou, B., Mural, R. V., Chen, X., Oates, M. E., Connor, R. A., Martin, Gregory B., Gough, J., Zeng, …
Plant Physiology.
173
:
1371–1390
Detecting the interaction of peptide ligands with plant membrane receptors
2017.
Hind, S. R., Hoki, J. S., Baccile, J. A., Boyle, P. C., Schroeder, Frank, Martin, Gregory B.
Current protocols in plant biology.
2
:
240–269
Generation of a Collection of Mutant Tomato Lines Using Pooled CRISPR Libraries
2017.
Jacobs, T. B., Zhang, N., Patel, D., Martin, Gregory B.
Plant Physiology.
174
:
2023–2037
Pseudomonas syringae pv. tomato Strains from New York Exhibit Virulence Attributes Intermediate Between Typical Race 0 and Race 1 Strains
2017.
Kraus, C. M., Mazo-Molina, C., Smart, C. D., Martin, Gregory B.
Plant Disease.
101
:
1442–1448
Detecting N-myristoylation and S-acylation of host and pathogen proteins in plants using click chemistry
2016.
Boyle, P. C., Schwizer, S., Hind, S. R., Kraus, C. M., Torre Diaz, S., He, B., Martin, Gregory B.
Plant Methods.
12
:
A novel method of transcriptome interpretation reveals a quantitative suppressive effect on tomato immune signaling by two domains in a single pathogen effector protein
2016.
Worley, J. N., Pombo, M. A., Zheng, Y., Dunham, D. M., Myers, C. R., Fei, Zhangjun, Martin, Gregory …
BMC Genomics.
17
:
Tomato receptor FLAGELLIN-SENSING 3 binds flgII-28 and activates the plant immune system
2016.
Hind, S. R., Strickler, S. R., Boyle, P. C., Dunham, D. M., Bao, Z., O'Doherty, I. M., Baccile, J. A…
Nature Plants.
2
:
16128–16128
iTAK: A Program for Genome-wide Prediction and Classification of Plant Transcription Factors, Transcriptional Regulators, and Protein Kinases
2016.
Zheng, Y., Jiao, C., Sun, H., Rosli, H. G., Pombo, M. A., Zhang, P., Banf, M., Dai, X., Martin, Greg…
Molecular Plant.
9
:
1667–1670
Natural Variation in Tomato Reveals Differences in the Recognition of AvrPto and AvrPtoB Effectors from Pseudomonas syringae
2016.
Kraus, C. M., Munkvold, K. R., Martin, Gregory B.
Molecular Plant.
9
:
639–649
High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
2016.
Jacobs, T. B., Martin, Gregory B.
Journal of visualized experiments : JoVE.
:
Pseudomonas syringae pv. tomato DC3000 Type III Secretion Effector Polymutants Reveal an Interplay between HopAD1 and AvrPtoB
2015.
Wei, H. L., Chakravarthy, S., Mathieu, J., Helmann, T. C., Stodghill, P., Swingle, Bryan M., Martin,…
Cell Host & Microbe.
17
:
752–762
Identification of a Candidate Gene in Solanum habrochaites for Resistance to a Race 1 Strain of Pseudomonas syringae pv. tomato
2015.
Bao, Z., Meng, F., Strickler, S. R., Dunham, D. M., Munkvold, K. R., Martin, Gregory B.
The Plant Genome.
8
:
0–0
The SGN VIGS Tool: User-Friendly Software to Design Virus-Induced Gene Silencing (VIGS) Constructs for Functional Genomics
2015.
Fernandez-Pozo, N., Rosli, H. G., Martin, Gregory B., Mueller, Lukas A.
Molecular Plant.
8
:
486–488
Transcriptomic analysis reveals tomato genes whose expression is induced specifically during effector-triggered immunity and identifies the Epk1 protein kinase which is required for the host response to three bacterial effector proteins
2014.
Pombo, M. A., Zheng, Y., Fernandez-Pozo, N., Dunham, D. M., Fei, Zhangjun, Martin, Gregory B.
Genome Biology.
15
:
Pto Kinase Binds Two Domains of AvrPtoB and Its Proximity to the Effector E3 Ligase Determines if It Evades Degradation and Activates Plant Immunity
2014.
Mathieu, J., Schwizer, S., Martin, Gregory B.
PLoS Pathogens.
10
:
e1004227–e1004227
Natural Variation for Responsiveness to flg22, flgII-28, and csp22 and Pseudomonas syringae pv. tomato in Heirloom Tomatoes
2014.
Veluchamy, S., Hind, S. R., Dunham, D. M., Martin, Gregory B., Panthee, D. R.
PLoS One 6.
9
:
e106119–e106119
Salmonella colonization activates the plant immune system and benefits from association with plant pathogenic bacteria
2013.
Meng, F., Altier, C., Martin, Gregory B.
Environmental Microbiology.
15
:
2418–2430
Transcriptomics-based screen for genes induced by flagellin and repressed by pathogen effectors identifies a cell wall-associated kinase involved in plant immunity
2013.
Rosli, H. G., Zheng, Y., Pombo, M. A., Zhong, S., Bombarely, A., Fei, Zhangjun, Collmer, Alan, Marti…
Genome Biology.
14
:
R139–R139
The Tomato Calcium Sensor Cbl10 and Its Interacting Protein Kinase Cipk6 Define a Signaling Pathway in Plant Immunity
2013.
de la Torre, F., Gutiérrez-Beltrán, E., Pareja-Jaime, Y., Chakravarthy, S., Martin, Gregory B., de…
THE PLANT CELL ONLINE.
25
:
2748–2764
Nucleic acids encoding proteins with pathogen resistance activity and plants transformed therewith
Greg Martin
Technology Area:Biotic Stress - Disease
US Patent/Application(s): 7,138,569
Publication: EMBO 2003
Nucleic acids encoding proteins with pathogen resistance activity and plants transformed therewith
Greg Martin
Technology Area:Biotic Stress - Disease
US Patent/Application(s): 6,653,533
Publication: EMBO 1997
Flagellin-sensing 3 (‘fls3’) protein and methods of use
Greg Martin
Technology Area:Biotic Stress - Disease
US Patent/Application(s): PCT/US2015/039520
Publication: Science 2016
Gene conferring disease resistance to plants by responding to an avirulence gene in plant pathogens
Greg Martin
Technology Area:Biotic Stress - Disease
US Patent/Application(s): 5,648,599
Publication: Science 1993
Increased Resistance to Race 1 Pseudomonas via Modulation of the Rph1
Greg Martin
Technology Area:Biotic Stress - Disease
US Patent/Application(s): 15/254,370
Bacterial effector proteins which inhibit programmed cell death
Greg Martin
Technology Area:Biotic Stress - Disease
US Patent/Application(s): 7,888,467
Publication: PNAS 2002