Carmen Catalá

Assistant Professor
Carmen Catalá
cc283@cornell.edu
Office/Lab: 315/316
Phone: 607-254-8694
Office/Lab: 315/316
Email: cc283@cornell.edu
Office Phone: 607-254-8694
Lab Phone: 607-254-8757
Affiliations: Section of Plant Biology / School of Integrative Plant Science / Cornell University
Research Overview

Our research program is focused on elucidating key questions related to auxin synthesis, translocation and the nature of auxin-regulated signaling networks during fruit development, using tomato as a model system.

During fruit set, the growth of an otherwise static ovary is stimulated after successful pollination and fertilization. After fertilization, tomato fruit growth is due primarily to cell division and later fruit growth continues mostly by cell expansion. At the end of the cell expansion period, the fruit has reached its final size and will start to ripen.

Auxin homeostasis during tomato fruit growth and development

Despite major advances made in recent years in many aspects of auxin metabolism, transport and signaling in vegetative tissues, the information about the nature and importance of these processes in fruit development and ripening of crop fruit species is very scarce. Moreover a recurring theme that emerges from all these studies is the lack of knowledge about the sources of auxin in fruit tissues, its biosynthetic pathway(s) and how auxin becomes distributed to fruit target tissues. Our research goal is to better understand the mechanisms by which auxin is produced and transported in tomato fruit and how these mechanisms are regulated to mediate cell and tissue specific growth and differentiation.

Expression of the DR5rev::mRFP auxin-responsive promoter in tomato ovaries and fruit. DR5 is a widely used auxin-responsive promoter and red fluorescence protein (RFP) signal therefore indirectly reflects auxin concentration. The top images correspond to flower buds six days before fertilization and show RFP fluorescence in the ovules confined to the micropylar pole of the embryo sac. Lower images correspond to six-day old fruit showing strong localized fluorescence in the seed funiculus.

Analysis of auxin levels or activity in different tomato tissues have revealed a dynamic pattern of tissue specific auxin accumulation throughout fruit development likely to be regulated by components of the auxin polar transport pathway. Critical components of auxin transport systems are the PIN and AUX/LAX protein families, which control cellular auxin efflux and influx respectively. Our studies have provided a transcriptional map for the PIN and AUX/LAX gene families of auxin transport facilitators in the tomato fruit, an important first step towards unraveling the complex network controlling auxin transport routes during fruit set and growth. Multiple PIN and AUX/LAX genes show both overlapping, and tissue-specific patterns of expression suggesting that the coordinated action of PIN and AUX proteins is required for establishing the adequate auxin pools and gradients controlling growth and differentiation in fruit tissues. We also seek to elucidate the mechanisms of IAA biosynthesis in tomato fruit and we are focusing on the tomato orthologs of the tryptophan aminotransferase of Arabidopsis (TAA1) which converts tryptophan into the IAA precursor indole-pyruvic acid and is a key enzyme contributing to IAA production in vivo.

The hypothesis underlying our research is that a tightly regulated spatial and temporal control of auxin levels during tomato fruit development is necessary to activate ovary growth upon fertilization and to coordinate cell expansion and differentiation during exponential fruit growth. We are testing this hypothesis by manipulating the gene expression of specific auxin transporters and auxin biosynthetic genes using fruit-specific promoters and analyzing the effect on fruit development and the dynamics of auxin distribution.

Cell-specific analysis of the tomato fruit transcriptome for the discovery of genes and networks regulating fruit development

One of the first objectives of this research, funded by the NSF Plant Genome Program, is to generate a comprehensive assessment of the cell specific transcript landscape of the developing tomato fruit using Laser Capture Microdissection (LCM) coupled with mRNA profiling by the Illumina platform.

We are mining the tissue-specific transcript datasets for genes associated with hormone signaling, synthesis and transport and with cell wall biosynthesis and modification processes. This non-targeted approach has the potential to dramatically increase the discovery of rare and cell-type specific transcripts and will help identify regulatory hormonal networks controlling auxin homeostasis, as well as new/novel components in the auxin biosynthetic, transport and response pathways. We are using this information to build a model integrating hormone regulated cell expansion and tissue growth and to identify pathways potentially critical to fruit set and growth.

Intern Projects

Investigating the molecular mechanisms underlying fruit set and development


Fruit development is a crucial process in the sexual reproduction of flowering plants and of critical importance for seed dispersal, plant fitness and agricultural yield. Fruit are complex organs which arise from the coordinated growth and development of floral tissues following pollination. Research in the Catala lab focuses on the molecular regulation of fruit formation and early development using tomato as a model system. We use molecular and genetic techniques to investigate the complex interplay of gene expression changes, signaling events, and hormonal activity, controlling fruit development. The lab also studies the effect of drought stress, an increasing problem in crop production, on tomato fruit set and growth. We are taking advantage of the genetic diversity of wild tomato species, to examine the molecular basis of adaptations to water stress as well as of other fruit quality traits.
Mechanisms regulating auxin action during fruit development
2014
Author(s):Pattison, R. J., Csukasi, F., and Catalá, C.
Physiologia Plantarum
151
62-72
View
Evaluating auxin distribution in tomato (Solanum lycopersicum) through an analysis of the PIN and AUX/LAX gene families
2012
Author(s):Pattison, R.J., and Catala, C.
Plant J.
70
585-598
View
Towards characterization of the glycoproteome of tomato (Solanum lycopersicum) fruit using Concanavalin A lectin affinity chromatography and LC-MALDI-MS/MS analysis
2011
Author(s):Catala, C., Howe, K.J., Hucko, S., Rose, J.K.C., and Thannhauser, T.W.
Proteomics
11
1530-1544
View
A Tomato Endo-ë’-1,4-glucanase, SlCel9C1, Represents a Distinct Subclass with a New Family of Carbohydrate Binding Modules (CBM49)
2007
Author(s):Urbanowicz, B.R., and Catala, C.
J. Biol. Chem.
282
12066-12074
View
A Tomato Endo-Β-1,4-glucanase, SlCel9C1, Represents a Distinct Subclass with a New Family of Carbohydrate Binding Modules (CBM49)
2007
Author(s):Urbanowicz, B.R., and Catala, C.
J. Biol. Chem.
282
12066-12074
View
Sample extraction techniques for enhanced proteomic analysis of plant tissues
2006
Author(s):Isaacson, T., Damasceno, C.M.B., Saravanan, R.S., He, Y., and Catala, C.
Nature Protocols
1
769-774
View
Characterization of a new xyloglucan endotransglucosylase/hydrolase (XTH) from ripening tomato fruit and implications for the diverse modes of enzymic action
2006
Author(s):Saladi, M., Rose, J.K.C., Cosgrove, D.J., and Catala, C.
Plant J.
47
282-295
View
The plot thickens: new perspectives of primary cell wall modification
2004
Author(s):Rose J.K., Saladi, M., and Catala, C.
Curr. Opin. Plant Biol.
7
296-301
View
Plant cell wall disassembly.
2003
Author(s):Rose J.K.C., and Catala, C.
In The Plant Cell Wall. Annual Plant Reviews Series (Ed. J.K.C. Rose, Pub). Blackwell Publishing
264-324
View
Characterization of a tomato xyloglucan endotransglycosylase gene that is down-regulated by auxin in etiolated hypocotyls. Plant Physiol., 127, 1180-1192
2001
Author(s):Catala, C., Rose, J.K.C., York, W.S., Albersheim, P., Darvill, A.G., and Bennett, A.B.
Plant Physiol.
127
1180-1192
View
Auxin-regulated genes encoding cell wall modifying proteins are expressed during early tomato fruit growth
2000
Author(s):Catala, C., Rose, J.K.C., and Bennett, A.B.
Plant Physiol.
122
527-534
View
Cloning and sequence analysis of TomCel8; a new plant endo-1,4-ë’-d-glucanase gene, encoding a protein with a putative carbohydrate binding domain (Accession No. AF098292)
1998
Author(s):Catala, C.
Plant Physiol.
118
1535
View
Cloning and sequence analysis of TomCel8; a new plant endo-1,4-Β-d-glucanase gene, encoding a protein with a putative carbohydrate binding domain (Accession No. AF098292)
1998
Author(s):Catala, C.
Plant Physiol.
118
1535
View
Auxin-induction and spatial localization of a novel endo-1,4-ë’-d-glucanase and a xyloglucan endotransglycosylase in tomato hypocotyls
1997
Author(s):Catala, C., Rose, J.K.C., and Bennett, A.B.
Plant J.
12
417-426
View
Auxin-induction and spatial localization of a novel endo-1,4-Β-d-glucanase and a xyloglucan endotransglycosylase in tomato hypocotyls
1997
Author(s):Catala, C., Rose, J.K.C., and Bennett, A.B.
Plant J.
12
417-426
View
Carbohydrate binding plant hydrolases which alter plant cell walls
Carmen Catala
Technology Area:Fruit Quality
US Patent/Application(s): 12/665,893
Publication: JBC 2007

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