First described in 1983, recombinant protein production in insect cells using BEVS, the technology has been used to produce thousands of proteins. The technology will continue to play a major role in our fight against emerging diseases.
Baculovirus-free methods using transient plasmid-based expression or stable transformation are well documented.
Transient or stable expression can be combined with infection using a non-recombinant “wild-type” baculovirus , to increase protein expression by transactivation.
Virus-free cell lines
The issue of adventious viruses in insect cell lines was recently reviewed. Trichoplusia ni cells are sucesptible to alphanodaviruses, but “unlike rhabdoviruses, alphanodaviruses are not generally considered to be mammalian pathogens”
First description of a virus-free sub-clone of HighFive cells:
The cell line, first believed to be from Ascalapha odorata (Ao38), was later identified as a Trichoplusia ni cell line and subl-clone of HighFive that emerged from a primary culture of A. odorata. It was renamed Tnao38. Erratum:
Protein glycosylation using BTI cell lines
The production of mammalianized glycoproteins in HighFive and Tnao38 cells can be achieved using the SweetBacTM approach