The Leica SP5 confocal system has eight visible laser lines provided by a multiline argon laser, a Diode-pumped solid state laser, and orange and red helium neon lasers. In addition, a UV diode laser provides excitation at 405 nm thus enabling the imaging of dyes such as DAPI and the blue fluorescent proteins

Laser Wavelength

• Near UV Diode: 405 nm
• Argon: 458 nm, 476 nm, 488 nm, 496 nm, 514 nm
• DPSS (Diode-Pumped Solid State): 561 nm
• HeNe Orange (Helium-Neon): 594 nm
• HeNe red (Helium-Neon): 633 nm

The laser lines are readily controlled by acousto-optical tunable filters (AOTF) and the combination of the acousto-optical beam splitter (AOBS), and the fully tunable spectral detectors provide maximal flexibility for the detection of dyes/fluorescent proteins with closely overlapping emission characteristics.

The three spectral confocal channels and two new HyD (hybrid detector) and a transmitted light channel permit the simultaneous capture of five fluorescent signals plus DIC.

This SP5 system is equipped with a conventional scanner and a high-speed “resonant” scanner capable of scanning up to twenty-five frames/second (512 x 512 format). The confocal system is attached to a Leica DM 6000 fully automated upright microscope with high numerical aperture oil and water immersion objectives and a SuperZ galvonometer-controlled stage.

Objectives

10X Dry

• Magnification / Aperture: 10X / 0.40
• Type: HC PL APO CS

20X Water

• Magnification / Aperture: 20X / 0.70
• Type: HC PL APO CS

40X Dry

• Magnification / Aperture: 40X / 0.85
• Type: HCX PL APO CS

40X Oil

• Magnification / Aperture: 40X / 1.25-0.75
• Type: HCX PL APO CS

63X Oil

• Magnification / Aperture: 63X / 1.40-0.60
• Type: HCX PL APO CS

63X Water

• Magnification / Aperture: 63X / 1.20
• Type: HCX PL APO

100X Oil

• Magnification / Aperture: 100X / 1.44
• Type: HCX PL APO

The microscope is equipped with a UV light source (Metal Halogenide) and five filter cubes (UV, green BP, green LP, red LP, and red BP):

Filter Cube A

• Excitation filter: BP 340-380
• Emission filter: LP 425

Filter Cube 13

• Excitation filter: BP 450-490
• Emission filter: LP 515

Filter Cube GFP

• Excitation filter: BP 450-490
• Emission filter: BP 500-550

Filter Cube N2,1

• Excitation filter: BP 515-560
• Emission filter: LP 590

Filter Cube N2,1

• Excitation filter: BP 546/12
• Emission filter: BP 580-620

Training is required, and the training fee is $110 per user. This covers a three-hour training session as well as assistance when using the microscope.

The usage fee is $28.50 per hour.

The Olympus SZX12 is a stereoscope with a long working distance and wide zoom range. It is equipped with DFPLFL 0.5X PF, DFPLAPO 1.2X, and DFPLFL 1.6X PF objectives coupled with 10 X eyepieces (Zoom range to 108X). At present the two parfocal objectives (0.5X and 1.6X) are mounted on a revolving nosepiece that allows easy switching between lenses and easy adjustments of focus. If you need to use 1.2X objective please contact us for the switch.

It is mounted on a transmitted light base and has dual fiber optics for reflected illumination. It is equipped with a color CCD camera connected to the ProgRes C14 acquisition software. A choice of filter cubes is available for fluorescence imaging:

Filter Cube UV (DAPI)

• Excitation filter: 350/50
• Emission filter: 460/50

Filter Cube BP Green (FITC)

• Excitation filter: 480/30
• Emission filter: 535/40

Filter Cube LP Green (GFP)

• Excitation filter: 470/40
• Emission filter: LP 500

Filter Cube Red (TRITC)

• Excitation filter: 540/25
• Emission filter: 605/55

This scope can be used free of charge following a free fifteen-minute training.

Training is required.

Leica DM5500 Epifluorescence Microscope is equipped with five objectives: 10X dry, 20X immersion, 40X dry, 63X water, and 63X oil. All objectives are equipped for bright field and Polarized imaging. DIC is currently unavailable on 10X and 40X. It has an automatic touch pad for lowering and raising the stage. A choice of filter cubes is available for fluorescence imaging. It is equipped with a color CCD camera Retiga-2000R connected to the Qcapture Pro 6.0 acquisition software.

Filter Cube UV A

• Excitation filter: BP 340-380
• Dichroic mirror: 400
• Emission filter: LP 425

Filter Cube Green 13

• Excitation filter: BP 450-490
• Dichroic mirror: 510
• Emission filter: LP 515

Filter Cube Green (GFP)

• Excitation filter: BP 450-490
• Dichroic mirror: 500
• Emission filter: BP 500-550

Filter Cube Red N2,1

• Excitation filter: BP 515-560
• Dichroic mirror: 580
• Emission filter: LP 590

This scope can be used free of charge following a free thirty-minute training.

Our free training covers all the basics of the microscope and image acquisition. Training takes about thirty minutes, and you can take it any day between 9 am and 1 pm (except Thursdays). Contact PCIC in advance to make sure that we are available to provide the training.

After an initial training, all instruments can be scheduled through the shared Microsoft Calendar.

To schedule an appointment for training with microscope use, or for questions, please contact us or call (607) 254-4436 between the hours of 9 am and 1 pm.

Olympus SZX12 Stereomicroscope

There is no usage charge for this microscope. Training is free.

Our free training covers all the basics of the microscope and image acquisition. Training takes about thirty minutes, and you can take it any day between 9 am and 1 pm (except Thursdays). Contact PCIC in advance to make sure that we are available to provide the training.

Leica DM5500 Epifluorescence Microscope

There is no usage charge for this microscope. Training is free.

Our free training covers all the basics of the microscope and image acquisition. Training is about thirty minutes, and you can take it any day between 9 am and 1 pm (except Thursdays). Contact PCIC in advance to make sure that we are available to provide the training.

Leica TCS SP5 Confocal Microscope

The hourly fee for the confocal microscope is $28.50 an hour. The training fee is $110 (approx. 2.5-3.0 hours general training).

The three-hour training session covers all the basics of the microscope and its software. If needed, we can tailor the training to specific areas of interest. Training is typically provided to up to three people at a time, and users do not need to bring samples.

After training is completed, free assistance is available during the following times:
– Monday (all day)
– Wednesday (9:00 AM–1:00 PM)
– Friday (all day)

To schedule a session, please contact pcic@cornell.edu. Note: A completed Confocal Authorization Form must be submitted at least 24 hours before the training session.