BTI LabNotes

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JoVE (Journal of Visualized Experiments) Videos

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Postdoctoral researcher Thomas Jacobs from Greg Martin’s lab, uses tomato hairy roots to demonstrate how multiple CRISPR vectors can be constructed in parallel in a single cloning reaction.

Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato

Graduate student André Velásquez from Greg Martin’s lab shows how to do Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato.

Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants

Research associate Suma Chakravarthy from Greg Martin’s lab, shows the procedure Assay for Pathogen-Associated Molecular Pattern (PAMP)-Triggered Immunity (PTI) in Plants.

Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis

BTI scientists recently exhibited their acting skills for the Journal of Visualized Experiments ( This video demonstrates how to mate and separate Chlamydomonas reinhardtii tetrads for genetic analysis and also how bugs interact with host plants.

The video by Xingshan Jiang explains the protocol for mating and tetrad separation of Chlamydomonas reinhardtii for genetic analysis.

 The White Cabbage Butterfly

The video by Martin de Vos explains how you can identify which plants are more resistant to tissue chewing caterpillars. He uses a no-choice set-up where caterpillars are forced to feed from a plant. After 7 days Martin compares the weight of the caterpillars. Some caterpillars will be full-grown, while others are still small. Small caterpillars mean that a plant is well defended against these attackers. The second experiment is a no choice experiment with white cabbage butterflies that are given a choice on which plant material to lay their eggs. The results show that these butterflies are attracted to plants with specific chemicals in the leaves.

 How To Set Up Aphid Artificial Diet Experiments

The video made by John Ramsey shows how some specialized green peach aphids (Myzus persicae) are able to feed from tobacco, whereas others cannot. This is likely because of the presence of nicotine. Nicotine is a toxic chemical that is not only present in cigarettes, but also in tobacco plants. John uses an artificial diet experiment to show that nicotine does not affect red aphids, but kills green aphids that belong to the same species. John is now trying to identify why these red aphids are able to withstand higher concentrations of nicotine in their food.

 Aphids That Transmit Plant Viruses

Cecilia also got her chance to shine in the spotlight of JoVE’s cameras. She studies aphids that transmit plant viruses and shows her viewers how to study virus transmission between infected plants, aphids, and non-infected plants. She uses a technique whereby aphids are injected with virus particles to determine their ability to transmit this virus.

Other Videos

Tomato: Decoded—Science of Genome Sequencing

Teachers and students can benefit from watching this eleven-minute video. BTI’s Dr. Jim Giovannoni speaks for a fourteen-nation consortium of geneticists and bioinformatics specialists who deciphered the sequence and location of the 35,000 genes of the tomato, an international food crop. This video focuses on the work done by American researchers, who explain the process of genome sequencing, and how a sequenced genome aids plant breeders in selecting precisely for desirable traits, including yield, shape, natural resistance to disease, and flavor. Credit: NBC Learn and the National Science Foundation.

Tomato Decoded

News Interviews with BTI Scientists



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