PCIC Manuals

Leica SP5 Confocal Microscope

The updated confocal manual version is available here: SP5_manual.

This manual is for the use of trained people only. If you have never used our confocal, you must contact PCIC to schedule a training.

Olympus Stereomicroscope

More information is provided in the facility.

Leica Confocal SP5 Application Notes

Please find a list of specific applications available with our confocal system in the following text. We can help you adjust your parameters: contact PCIC.

How to (choose from the following links) . . .

• Limit the crosstalk between your fluorophores
• Zoom in the live window and other usefull live tools
• Draw a region of interest
• Scan only a region of interest
• Perform a lamda scan
• Perform a sequential scan
• Enhance very weak signals
• Compensate for the loss of intensity along the z axis during an XYZ stack
• Export data and reports
• Perform a time-lapse experiment
• Use the FRET SE or FRET photobleaching wizards. If you never performed FRET, please contact PCIC.